---
title: "Alzheimer’s disease single-neuron whole-genome sequencing – Miller 2022"
id: "3581"
type: "sample_set"
slug: "snd10029"
published_at: "2022-05-11T16:08:05+00:00"
modified_at: "2022-06-01T18:07:06+00:00"
url: "https://dss.niagads.org/sample-sets/snd10029/"
markdown_url: "https://dss.niagads.org/sample-sets/snd10029.md"
excerpt: "This study performed whole-genome sequencing of single neurons isolated from prefrontal cortex (PFC) and hippocampus CA1 (HC) of postmortem human brain of individuals with Alzheimer’s disease (AD) (Braak stage V-VI) or non-disease control, along with bulk whole-genome sequencing performed on..."
---

## Description

This study performed whole-genome sequencing of single neurons isolated from prefrontal cortex (PFC) and hippocampus CA1 (HC) of postmortem human brain of individuals with Alzheimer’s disease (AD) (Braak stage V-VI) or non-disease control, along with bulk whole-genome sequencing performed on each individual as a reference genome for variant calling. Single neuron genomes were amplified using multiple-displacement amplification (MDA) or primary template-directed amplification (PTA).

## Cohorts

- [Massachusetts Alzheimer’s Disease Research Center (MassADRC)](https://dss.niagads.org/cohorts/massachusetts-alzheimers-disease-research-center/)
- [NIH Neurobiobank/University of Maryland (UMB-BTB)](https://dss.niagads.org/cohorts/nih-neurobiobank-university-of-maryland/)

## Sex

### Total number of samples: 29

Female1758.6 %

Male1241.4 %

## Diagnosis

### Total number of samples: 29

| AD |  |  |
| --- | --- | --- |
| Control | 20 | 69.0% |
| Case | 9 | 31.0% |