---
title: "Alzheimer’s disease single-neuron whole-genome sequencing – Miller 2022"
id: "3588"
type: "study"
slug: "sa000022"
published_at: "2022-05-11T16:04:30+00:00"
modified_at: "2022-11-14T20:00:08+00:00"
url: "https://dss.niagads.org/studies/sa000022/"
markdown_url: "https://dss.niagads.org/studies/sa000022.md"
excerpt: "This study performed whole-genome sequencing of single neurons isolated from prefrontal cortex (PFC) and hippocampus CA1 (HC) of postmortem human brain of individuals with Alzheimer’s disease (AD)(Braak stage V-VI) or non-disease control, along with bulk whole-genome sequencing performed on each..."
---

## Description

This study performed whole-genome sequencing of single neurons isolated from prefrontal cortex (PFC) and hippocampus CA1 (HC) of postmortem human brain of individuals with Alzheimer’s disease (AD)(Braak stage V-VI) or non-disease control, along with bulk whole-genome sequencing performed on each individual as a reference genome for variant calling. Single neuron genomes were amplified using multiple-displacement amplification (MDA) or primary template-directed amplification (PTA).

## PI

Michael Miller  
 Brigham and Women’s Hospital

## Associated Datasets

- [NG00121 - Alzheimer’s disease single-neuron whole-genome sequencing - Miller 2022](https://dss.niagads.org/datasets/ng00121/) Single-neuron amplified genome libraries were sequenced (WGS) on Illumina instruments and aligned to the human reference genome GRCh37. Linked-read analysis (LiRA) and SCAN-SNV were used to identify single-cell somatic mutations.… [Learn more](https://dss.niagads.org/datasets/ng00121/)

## Cohorts

- [Massachusetts Alzheimer’s Disease Research Center (MassADRC)](https://dss.niagads.org/cohorts/massachusetts-alzheimers-disease-research-center/) The Massachusetts Alzheimer’s Disease Research Center (MADRC) is a P30 research center funded by the National Institute on Aging. Our center has been at the forefront of dementia research, treatment… [Learn more](https://dss.niagads.org/cohorts/massachusetts-alzheimers-disease-research-center/)
- [NIH Neurobiobank/University of Maryland (UMB-BTB)](https://dss.niagads.org/cohorts/nih-neurobiobank-university-of-maryland/) The National Institutes of Health have established and funded a network of six Brain and Tissue Banks referred to as the NIH NeuroBioBank. Each of the banks collects and distributes… [Learn more](https://dss.niagads.org/cohorts/nih-neurobiobank-university-of-maryland/)

## Grants

NIH K08 AG065502

Doris Duke Charitable Foundation Clinical Scientist Development Award 2021183

## Acknowledgement

### Acknowledgment statement for any data distributed by NIAGADS:

Data for this study were prepared, archived, and distributed by the National Institute on Aging Alzheimer's Disease Data Storage Site (NIAGADS) at the University of Pennsylvania (U24-AG041689), funded by the National Institute on Aging.

### For investigators using Alzheimer’s disease single-neuron whole-genome sequencing – Miller 2022 data:

NIH Sponsoring Institute: NIA

Grant funding: NIH K08 AG065502

Doris Duke Charitable Foundation Clinical Scientist Development Award 2021183