Description

This dataset contains brain quantitative trait locus (xQTL) summary statistics across six molecular phenotypes: expression quantitative trait loci (eQTL), histone acetylation quantitative trait loci (haQTL), DNA methylation quantitative trait loci (mQTL), protein quantitative trait loci (pQTL), splicing quantitative trait loci (sQTL), and single-nucleus expression quantitative trait loci (snuc-eQTL). The data were generated from four cohorts (ROSMAP, MSBB, Knight-ADRC, MiGA NBB, MiGA MSSM) and 20 brain cell types and regions, including microglia, monocytes, and multiple cortical areas. xQTLs were derived using a standardized Alzheimer’s Disease Sequencing Project Functional Genomics (ADSP FunGen-AD) pipeline with stringent quality control, followed by harmonization to a common set of genomic references to maximize accessibility and interoperability.

Unlike previous xQTL resources, all summary statistics in this atlas provide consistent information on effect alleles, allele frequencies, and effect directions in uniform file formats, and include both full and significance-filtered result sets. This consistency is intended to remove common barriers to downstream applications such as colocalization, Mendelian randomization, and integrative fine-mapping, where heterogeneous formats and partial releases have been a major limitation.

The atlas also extends cis-testing windows using brain topologically associating domains (TADs), yielding additional xQTLs beyond conventional fixed 1 megabase (Mb) windows and supporting more complete detection of regulatory variants. Variants and non-genic molecular features are annotated with overlapping or nearest genes to simplify gene-centric investigation.

The ADSP Functional Genomics xQTL Atlas provides fully quality-controlled xQTL results, high-quality significant xQTL associations using Benjamini–Hochberg and Hierarchical Multiple Testing (HMT) adjustments, and single-context fine-mapping results generated via SuSIE. All results are in standardized gzipped browser extensible data (BED–like) formats that are also browsable through the companion web portal at https://xqtl.niagads.org/.

Available Filesets

NameAccessionLatest ReleaseDescription
ADSP FunGen xQTL Atlas (open access)fsa000172NG00184.v1QTL Summary Statistics

View the File Manifest for a full list of files released in this dataset.

 

ADSP FunGen xQTL Atlas Download Guide

Most users should download a packaged .tar archive rather than individual files. Archives are named as:

ADSP_FunGen_xQTL.v1.<QTL type>.<result type>.tar

Example: ADSP_FunGen_xQTL.v1.pQTL.hmt_significant.tar

User goalRecommended result typeUse case
Start with high-confidence xQTL associationshmt_significantBest default choice for most users
Use a broader significant association setbh_significantMore inclusive than HMT; still FDR-filtered
Access all tested associationsallComplete summary statistics for custom analyses
Prioritize likely causal variantssingle_context_finemapping_cs95Fine-mapped variants in 95% credible sets
Review all fine-mapping outputsingle_context_finemapping_allAdvanced fine-mapping analyses

Choose the QTL type based on the molecular layer of interest:

QTL typeMolecular layer
eQTLBulk gene expression
snuc-eQTLCell-type-specific single-nucleus expression
sQTLAlternative splicing
pQTLProtein abundance
mQTLDNA methylation
haQTLH3K9ac histone acetylation

Recommended starting point: download <QTL type>.hmt_significant.tar for association results, or <QTL type>.single_context_finemapping_cs95.tar for fine-mapping results.

Each .tar archive includes harmonized .bed.gz files, .bed.gz.tbi index files, metadata, and a manifest.